A source of error in an autoanalyzer determination of serum iron.
نویسندگان
چکیده
An AutoAnalyzer method for serum iron was found to be giving erroneously high values owing to a Donnan equilibrium effect on the rate of dialysis of ferrous ions. The addition of neutral salt to the reagent corrected the deficiency. The validity of other assays on the AutoAnalyzer that measure dialyzable ions in the presence of relatively high concentrations of protein should be verified in view of this finding.
منابع مشابه
Low Dose, Short-Term Iron Supplementation in Female Blood Donors of Childbearing Age: a Randomized, Double-Masked, Placebo-Controlled Study
: Iron supplementation for blood donors is a controversial concept. However, to maintain regular blood donors, as a source of blood supply, the present paradigm is not appropriate and dose not prevent harms to blood donors. Methods: A randomized, double-masked, placebo-controlled study, was conducted by enrolling 95 female regular blood donors of childbearing age (18-49 years). The participants...
متن کاملAn autoanalyzer procedure for serum iron and total iron-binding capacity, with use of ferrozine.
An AutoAnalyzer method has been developed in which the recently introduced reagent for iron, 3. (2.pyridyl).5,6.bis(4.phenylsulfonic acid).1,2,4.triazine (“ferrozine”), is used. With the Technicon colorimeter or Gilford 300-N spectrophotometer in use, sampling rates may be 30/h or 50/h, respectively. Sample volume is 0.8 ml, and measurements are made at 560 nm. A procedure for measuring total i...
متن کاملDetermination of serum calcium by use of an AutoAnalyzer I-II hybrid.
A hybridized system is described for the continuousflow determination of serum calcium. The analysis is based on the cresolphthalein colorimetric procedure as described for use with the SMA 12/60 (Technicon Corp.). The hydraulic features of the AutoAnalyzer II are used with modules of the AutoAnalyzer 1. This combination gives both the ease of operation and hydraulic improvements inherent in th...
متن کاملInfluence of iron saturation as a possible source of error in the immunoturbidimetric determination of serum transferrin.
The serum transferrin concentration is usually overestimated when assessed as total iron binding capacity, because iron binds not only to transferrin but also, non-specifically, to other serum proteins [l-3]. Transferrin concentration can be measured directly by immunochemical methods including: radial immunodiffusion [ 1,2,4-61, immunonephelometric assay [6,7] and immunoturbidimetric assay [8,...
متن کاملUseof an Anion-ExchangeColumnto Determine SerumIron-BindingCapacity1
A rapid procedure is described for determining total iron-binding capacity (TIBC) by use of a small, disposable resin column. Serum is saturated with excess ferric citrate, and iron not bound to protein is then removed by passing the mixture through the column of anion-exchanger. Transferrin-bound iron in the effluent, the TIBC, is determined colorimetrically (AutoAnalyzer), with 2,4,6-tripyrid...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 13 2 شماره
صفحات -
تاریخ انتشار 1967